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The technical solution of using Angel yeast extract FM902 to replace brewer’s yeast extract

May 8, 2019

By Yinqiang

Angel yeast extract can be used well to replace brewer's yeast extract in fermentation industry

1. Advantages of this solution

        - Reduce the overall cost of production;

        - Improve the user's current fermentation level;

          - Improve fermentation stability;

        - Raw materials are traceable;

          - Easy to use and save manpower;

        - Conducive to on-site hygiene management of the fermentation plant, reducing pollution sources;

         - The color of the substitute product is light, which is conducive to refining treatment;

         - Easy to save and reduce storage occupation;

         - Solve the problem that beer yeast extract does not meet the food and drug safety certification requirements;

         - No risk of Bacillus thermophilus contamination.

2. Introduction of Angel yeast extract FM902

    This product uses high-quality baker’s yeast as raw material with advanced production technology, stable product quality, rich nutrients and high effective utilization rate, meeting various safety certification requirements for food and medicine for raw materials.

     

3. Application and replacement ratio of using FM902 to replace brewer’s yeast extract

                    Application

                    Replacement ratio

                    Benefit

                    Vitamin C

                    0.3-0.45:1

                    Reduce production costs and improve the certification level of VC products themselves

                    Avermectin

                    0.35-0.45:1

                    Maintain the original process fermentation titer and reduce production cost

                    Long chain dibasic acid

                    0.3-0.4:1

                    Improve process stability and reduce costs

                    Xanthan gum

                    0.6:1

                    Increase seed bacteria concentration 5-7 times, shorten commodity fermentation time

                    Threonine

                    0.6:1

                    Increase acid production by 8%, shorten fermentation time and increase conversion rate

                    Corticosteroid

                    0.3-0.4:1

                    Reduce production costs, reduce the chance of fermentation and increase production stability

    * According to the design principle of total nitrogen balance, the use of FM902 instead of brewer's yeast extract should increase the amount of cheap nitrogen source such as corn syrup and soybean meal hydrolyzate.

     

     

4. Replacement cost assessment

     

    a. Comprehensive comparison of the performance and extraction effect of brewer’s yeast and baker’s yeast

                     

                    Brewer’s yeast extract

                    Baker’s yeast extract

                    Raw materials

                    Waste yeast from brewer’s plant

                    purified cultured high protein yeast

                    Stability of raw materials

                    Not guaranteed

                    Good

                    Main raw materials

                    Wort

                    Molasses

                    Hops

                    add hops, the taste is extremely difficult, the color is dark.

                    Non hops

                    Status of cells

                    Dead cells, weak cells, uneven,

                    High viable cell rate, strong, uniform

                    Cell wall

                    Thicker

                    Thinner

                    Protein content

                    Lower

                    Higher

                    Endogenous enzyme activity

                    Lower

                    Higher

                    Yield(%)

                    Lower

                    Higher

                    Sensory quality

                    Dark color and smelly

                    light color, rich fragrance

                    Total and amino nitrogen content

                    Lower

                    Higher

                    Stability

                    Difficult to control

                    Strict control

                    Allergen

                    Malt, rice, etc.

                    Non

                    Contamination of Bacillus thermophilus

                    Contain Bacillus thermophilus

                    Not contain

                    Kosher and Halal

                    Generally no Kosher and Halal

                    Kosher and Halal certified

                    Market range

                    Low-end market

                    Widely used

    Note: Yeast extract yield (%) = concentrated yeast extract dry matter / raw yeast dry weight

     

    b. Comparison of product analysis data

                     

                    Brewer’s

                    yeast extract

                    Angel yeast extract LM800

                    Angel yeast extract FM902

                    Free amino acid

                    Free amino acid

                    Free amino acid

                    Aspartic acid (Asp)

                    0.849

                    1.384

                    1.705

                    Threonine (Thr)

                    0.881

                    1.182

                    1.788

                    Serine (Ser)

                    0.796

                    0.088

                    1.662

                    Glutamate (Glu)

                    2.537

                    3.998

                    5.363

                    Glycine (Gly)

                    0.573

                    0.652

                    0.936

                    Alanine (Ala)

                    1.821

                    2.940

                    3.635

                    Cystine (Cys)

                    0

                    0.000

                    0.000

                    Valine (Val)

                    1.207

                    1.463

                    2.167

                    Methionine (Met)

                    0.401

                    0.378

                    0.654

                    Isoleucine (Ile)

                    1.005

                    1.254

                    1.734

                    Leucine (Leu)

                    1.959

                    2.083

                    3.352

                    Tyrosine (Tyr)

                    0.924

                    1.887

                    1.819

                    Phenylalanine (Phe)

                    1.015

                    1.464

                    1.904

                    Lysine (Lys)

                    0.911

                    1.138

                    1.672

                    Histidine (His)

                    0.164

                    0.291

                    0.521

                    Arginine (Arg)

                    0.787

                    1.051

                    1.550

                    Proline (Pro)

                    1.077

                    0.440

                    0.677

                    Total

                    16.907

                    21.6922

                    31.14017

     

     

5. Bacillus thermophilus detection of brewer's yeast extract

    Screening method of Bacillus thermophilus in brewer’s yeast extract

    1. The components were suspended in distilled water, allowed to stand for 5 min, mixed uniformly, heated, and continuously stirred for 1 min, and placed in a triangular flask at 121 ° C for autoclaving for 40 min.

    2. Sterly weigh the appropriate amount of the sample in a clean bench, add it to the pre-sterilized distilled water (keeping the temperature of the water at 40-45 ° C), and shake the container quickly to mix.

    3. Immediately pipette the suspension with a pipette and add to a flask containing sterile DTA (solution temperature 50-60 ° C).

    4. Quickly shake the flask and mix again. Immediately after mixing, place the flask in a pre-heated autoclave, heat to 5 lbs. pressure (108.4 °C) for 10 min, then quickly vent the autoclave. Immediately remove the flask from the autoclave and cool.

    5. Gently shake the Erlenmeyer flask and evenly dispense the medium from the vial into the sterilized plate. The agar solidifies.

    6. The plate was inverted and cultured in an incubator at 55 ± 2 ° C for 72 hours. Pick a single clone of different cell morphology to the bevel.

  

About Angel Microbial Nutrition

    Angel YE, rich in protein, amino acids, peptides, nucleotides, B vitamins and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency and stable quality, Angel YE are widely applied in the biological laboratories and industrial-scale fermentation, promoting the product safety and technical progress in the global fermentation industry.
     
About Angel:
    Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 150 countries and regions.
     
     
    Press contact:
    ANGEL YEAST CO., LTD
    Address: 168 Chengdong Avenue, Yichang, Hubei 443003, P. R.China
    Tel:    +86-717-6369520, 6369558
    Fax:   +86-717-6370680
    email: aie@angelyeast.com
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