Blog

The technical solution of using Angel yeast extract FM902 to replace brewer’s yeast extract

May 8, 2019

By Yinqiang

Angel yeast extract can be used well to replace brewer's yeast extract in fermentation industry

1. Advantages of this solution

    - Reduce the overall cost of production;

    - Improve the user's current fermentation level;

      - Improve fermentation stability;

    - Raw materials are traceable;

      - Easy to use and save manpower;

    - Conducive to on-site hygiene management of the fermentation plant, reducing pollution sources;

     - The color of the substitute product is light, which is conducive to refining treatment;

     - Easy to save and reduce storage occupation;

     - Solve the problem that beer yeast extract does not meet the food and drug safety certification requirements;

     - No risk of Bacillus thermophilus contamination.

2. Introduction of Angel yeast extract FM902

This product uses high-quality baker’s yeast as raw material with advanced production technology, stable product quality, rich nutrients and high effective utilization rate, meeting various safety certification requirements for food and medicine for raw materials.

 

3. Application and replacement ratio of using FM902 to replace brewer’s yeast extract

Application

Replacement ratio

Benefit

Vitamin C

0.3-0.45:1

Reduce production costs and improve the certification level of VC products themselves

Avermectin

0.35-0.45:1

Maintain the original process fermentation titer and reduce production cost

Long chain dibasic acid

0.3-0.4:1

Improve process stability and reduce costs

Xanthan gum

0.6:1

Increase seed bacteria concentration 5-7 times, shorten commodity fermentation time

Threonine

0.6:1

Increase acid production by 8%, shorten fermentation time and increase conversion rate

Corticosteroid

0.3-0.4:1

Reduce production costs, reduce the chance of fermentation and increase production stability

* According to the design principle of total nitrogen balance, the use of FM902 instead of brewer's yeast extract should increase the amount of cheap nitrogen source such as corn syrup and soybean meal hydrolyzate.

 

 

4. Replacement cost assessment

 

a. Comprehensive comparison of the performance and extraction effect of brewer’s yeast and baker’s yeast

 

Brewer’s yeast extract

Baker’s yeast extract

Raw materials

Waste yeast from brewer’s plant

purified cultured high protein yeast

Stability of raw materials

Not guaranteed

Good

Main raw materials

Wort

Molasses

Hops

add hops, the taste is extremely difficult, the color is dark.

Non hops

Status of cells

Dead cells, weak cells, uneven,

High viable cell rate, strong, uniform

Cell wall

Thicker

Thinner

Protein content

Lower

Higher

Endogenous enzyme activity

Lower

Higher

Yield(%)

Lower

Higher

Sensory quality

Dark color and smelly

light color, rich fragrance

Total and amino nitrogen content

Lower

Higher

Stability

Difficult to control

Strict control

Allergen

Malt, rice, etc.

Non

Contamination of Bacillus thermophilus

Contain Bacillus thermophilus

Not contain

Kosher and Halal

Generally no Kosher and Halal

Kosher and Halal certified

Market range

Low-end market

Widely used

Note: Yeast extract yield (%) = concentrated yeast extract dry matter / raw yeast dry weight

 

b. Comparison of product analysis data

 

Brewer’s

yeast extract

Angel yeast extract LM800

Angel yeast extract FM902

Free amino acid

Free amino acid

Free amino acid

Aspartic acid (Asp)

0.849

1.384

1.705

Threonine (Thr)

0.881

1.182

1.788

Serine (Ser)

0.796

0.088

1.662

Glutamate (Glu)

2.537

3.998

5.363

Glycine (Gly)

0.573

0.652

0.936

Alanine (Ala)

1.821

2.940

3.635

Cystine (Cys)

0

0.000

0.000

Valine (Val)

1.207

1.463

2.167

Methionine (Met)

0.401

0.378

0.654

Isoleucine (Ile)

1.005

1.254

1.734

Leucine (Leu)

1.959

2.083

3.352

Tyrosine (Tyr)

0.924

1.887

1.819

Phenylalanine (Phe)

1.015

1.464

1.904

Lysine (Lys)

0.911

1.138

1.672

Histidine (His)

0.164

0.291

0.521

Arginine (Arg)

0.787

1.051

1.550

Proline (Pro)

1.077

0.440

0.677

Total

16.907

21.6922

31.14017

 

 

5. Bacillus thermophilus detection of brewer's yeast extract

Screening method of Bacillus thermophilus in brewer’s yeast extract

1. The components were suspended in distilled water, allowed to stand for 5 min, mixed uniformly, heated, and continuously stirred for 1 min, and placed in a triangular flask at 121 ° C for autoclaving for 40 min.

2. Sterly weigh the appropriate amount of the sample in a clean bench, add it to the pre-sterilized distilled water (keeping the temperature of the water at 40-45 ° C), and shake the container quickly to mix.

3. Immediately pipette the suspension with a pipette and add to a flask containing sterile DTA (solution temperature 50-60 ° C).

4. Quickly shake the flask and mix again. Immediately after mixing, place the flask in a pre-heated autoclave, heat to 5 lbs. pressure (108.4 °C) for 10 min, then quickly vent the autoclave. Immediately remove the flask from the autoclave and cool.

5. Gently shake the Erlenmeyer flask and evenly dispense the medium from the vial into the sterilized plate. The agar solidifies.

6. The plate was inverted and cultured in an incubator at 55 ± 2 ° C for 72 hours. Pick a single clone of different cell morphology to the bevel.

  

About Angel Microbial Nutrition

Angel YE, rich in protein, amino acids, peptides, nucleotides, B vitamins and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency and stable quality, Angel YE are widely applied in the biological laboratories and industrial-scale fermentation, promoting the product safety and technical progress in the global fermentation industry.
 
About Angel:
Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 150 countries and regions.
 
 
Press contact:
ANGEL YEAST CO., LTD
Address: 168 Chengdong Avenue, Yichang, Hubei 443003, P. R.China
Tel:    +86-717-6369520, 6369558
Fax:   +86-717-6370680
email: aie@angelyeast.com
Share on: