Plant protein hydrolysates are products Produced by enzymatic hydrolysis, concentration, and drying of plant proteins such as soybean, wheat, and pea. Rich in polysaccharides, amino acids, peptides, vitamins, and trace elements, these hydrolysates are widely used in mammalian cell culture for producing antibodies, recombinant protein drugs, and vaccines. Their benefits include a complete amino acid profile, excellent thermal stability, and the Freedom from animal-derived risks.
Key mechanisms of action include:
1. Providing metabolic nitrogen sources and growth co-factors to promote high-density cell proliferation.
2. Optimizing energy metabolism pathways to enhance antibody titers.
3. Stabilizing the viral particles by peptide components to improve vaccine production efficiency.v
In animal cell culture, Angel has developed a series of plant protein hydrolysates with tailored nutritional profiles by optimizing production processes for different protein sources. Validated by extensive experiments and customer feedback, Angel’s products (PU040, PU041, PU042, PU046, and PU020) demonstrated significant potential in various cell culture applications.
·PU040: A standard soybean hydrolysate with balanced nutrients, suitable for CHO, HEK293, and other cell lines.
·PU041: Rich in soybean oligosaccharides, ideal for adherent cells (e.g., C2C12, DF-1, Vero). May substitute serum to support cell proliferation at optimal concentrations. (see Fig. 1)
·PU042: High ammonia nitrogen and amino acid content, tailored for insect cell lines (e.g., SF9, Highfive). It may boosts cell density, viral titers, and protein expression during serial passaging (see Fig. 2).
·PU046: A high-protein soybean hydrolysate with elevated nitrogen levels, suitable for BHK21, MDCK, and HEK293.
Figures:
Fig1. PU041 enhances adherent cell proliferation
Fig2. PU042 improves SF9 cell density and viral titers
Major Benefits:
·Nutritional Support: Amino acids and peptides to support robust cell growth.
·Metabolic Activation: Polysaccharides may enhance metabolic activity and product expression.
·Process Stability: Zwitterionic peptides (e.g., Glu-Lys) stabilizatoin pH (±0.1 vs. ±0.5).
About Angel Fermentation Nutrients：
Angel Fermentation Nutrients, rich in protein, amino acids, peptides, nucleotides, B vitamins, and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency, and stable quality, Angel YE are widely applied in biological laboratories and industrial-scale fermentation, promoting product safety and technical progress in the global fermentation industry.
About Angel:
Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 160 countries and regions.
Press contact:
email: tangshuo@angelyeast.com
