July 17, 2026

Zhong Zheng

In the fields of microbial fermentation and recombinant protein expression, animal-derived Acid Hydrolysate of Casein is a classic nitrogen source. However, its high ash content, high chloride ion levels, inherent risks associated with animal-derived raw materials, and inconsistent batch performance have long hindered precise process control and compliance upgrades. To address these drawbacks, we have developed a novel yeast hydrolysate. It not only serves as an animal-source-free alternative but also delivers breakthrough performance in key functional indicators.

· High degree of hydrolysis: Produced via deep enzymatic hydrolysis to deliver a balanced and complete amino acid profile, with a hydrolysis degree of 65%. Small peptides and amino acids below 1000 Da account for over 99% of total amino acids, matching the performance of Acid Hydrolysate of Casein.

· Low ash content: Chloride ion content controlled below 2.0%, total ash below 15%, eliminating ion inhibition and residual interference effects.

· Excellent growth-promoting activity: Exhibits robust growth stimulation for a wide range of microorganisms with broad universal applicability; significantly boosts recombinant protein expression yields.

· Clean and safe formulation: Animal-free with minimal batch-to-batch variation, featuring lower costs and stable raw material supply.

• Physicochemical Parameter Comparison

1. Physicochemical Indicators

Note: Degree of Hydrolysis = Amino Acid Nitrogen ÷ Total Nitrogen

2. Molecular Weight Distribution of Peptides

Abbreviation explanation: FAA = Free Amino Acids; TAA = Total Hydrolyzed Amino Acids

• Microbial Growth Promotion Performance

Typical medium composition during the tests:

Competitor：Acid Hydrolysate of Casein 10g/L + yeast extract FM888 5g/L + sodium chloride 10g/L

CM10：Yeast protein hydrolysate CM10 10g/L + yeast extract FM888 5g/L + sodium chloride 10g/L

• Recombinant Protein Expression Enhancement Performance

Typical medium composition during the tests:

Competitor：Acid Hydrolysate of Casein 10g/L + yeast extract FM888 5g/L + sodium chloride 10g/L

CM10：Yeast protein hydrolysate CM10 10g/L + yeast extract FM888 5g/L + sodium chloride 10g/L

About Angel Fermentation Nutrients：

Angel Fermentation Nutrients, rich in protein, amino acids, peptides, nucleotides, B vitamins, and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency, and stable quality, Angel YE are widely applied in biological laboratories and industrial-scale fermentation, promoting product safety and technical progress in the global fermentation industry.

About Angel:

Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 160 countries and regions.

Press contact:

email: yef@angelyeast.com