Blog

Selection of Nitrogen Source in Cultivation of Lactic Acid Bacteria

Feb 19, 2021

By Xiong Changwu

Lactic Acid Bacteria(LAB) are more and more widely used in food processing, dietary supplements and functional foods with tens of millions of strains involved. Generally speaking, lactic acid bacteria are difficult to cultivate, but the physiological characteristics of different lactic acid bacteria are significantly different. Some strains have strong synthesis and catabolism ability with low nutritional requirements, while other strains lack some metabolic pathways and have strict nutritional requirements.

6577b0f2e97c91d0.jpg


Type

Physiological characteristics

Strains

LAB

(Easy to cultivate)

Rich in peptidases, can synthesize certain amino acid, vitamins and growth factors independently

Lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus paracasei, Bifidobacterium lactis, etc.

LAB

(Hard to cultivate)

Weak synthesis and catabolism ability, sensitive to various growth factors which need to be obtained directly from outside

Lactobacillus acidophilus, Lactobacillus reuteri, Bifidobacterium longum, Bifidobacterium infantis, etc.


Whether the nutritional requirements of lactic acid bacteria are severe or not is manifested in whether the change of the nitrogen source in the medium and the related growth factors have a significant impact on the biomass in the cultivation of lactic acid bacteria. Generally, nitrogen sources with different nutritional characteristics have no significant effect on lactic acid bacteria that are easier to cultivate.

1-02.jpg

It can be seen from some of the strains we tested that changes in nitrogen source have no significant effect on the biomass of Lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus paracasei and other strains under the same culture conditions. For other strains such as Lactobacillus acidophilus, Lactobacillus bulgaricus, yeast extracts with different nutritional characteristics have obvious effects, and the fluctuation of biomass can reach more than 50%.

2.jpg

It can be seen from the test results that Bifidobacterium lactis is less affected by yeast extracts with different nutritional characteristics, while Bifidobacterium longum is more affected by yeast extracts with different nutritional characteristics.


Based on the cultivation results of lactic acid bacteria under the same conditions, it can be seen that for the easy-to-cultivable lactic acid bacteria, Angel's nutrient-enriched yeast extract FM985 and other grades, with balanced nutrients such as protein and free amino acids, can perfectly meet the growth of such lactic acid bacteria, while for Lactobacillus acidophilus and Bifidobacterium longum, Angel's nutrient-enriched yeast extract FM502, rich in related growth factors, is more conducive to biomass.

In addition to the impact of biomass, it can be found in our application research and tests that yeast extract FM502, which is rich in special nutrients, also has a certain improvement in the shelf life of lactic acid bacteria.

3.png4.png

The Lactobacillus acidophilus cultivated by yeast extract FM502 is stored for different periods of time at 25°C, compared with other yeast extracts, the number of viable bacteria has a higher survival rate.

Different lactic acid bacteria have different nutritional characteristics requirements. According to comprehensive application tests and customer feedback, using Angel's yeast extract FM985, FM502 and related yeast extracts solely or together can meet the growth needs of lactic acid bacteria with obtaining higher biomass and longer shelf life. The specific recommendations of different yeast extracts are as follows:

5-02.jpg


2994f7bd5f561580.jpg


Published by Xiong Changwu

R&D expert of Protein Nutrition and Flavoring technology center


About Angel Microbial Nutrition:

Angel YE, rich in protein, amino acids, peptides, nucleotides, B vitamins and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency and stable quality, Angel YE are widely applied in the biological laboratories and industrial-scale fermentation, promoting the product safety and technical progress in the global fermentation industry.

About Angel:

Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 150 countries and regions.


Press contact:

ANGEL YEAST CO., LTD

Address: 168 Chengdong Avenue, Yichang, Hubei 443003, P. R.China

Tel: +86-717-6369227

Fax: +86-717-6368757

email: aie@angelyeast.com

Share on:

Related tags: