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Customized development and application of Angel culture media

By Chen Lin

Jul 15, 2021


Microbial nutrition business of Angel Yeast Co., Ltd has developed in bio-fermentation industry for over ten years, and established a professional production line of culture medium with an annual output of 10,000 tons. It has introduced customized culture media for animal bacterial vaccines, reagent testing, vinegar fermentation, micro-ecologic and edible fungi to achieve the transition from raw material for culture medium to culture medium itself. Angel culture media are highly recognized by industry customers due to its convenient use, stable quality and cost effective performance.

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1. Introduction of Angel animal bacterial vaccine medium

In recent years, many countries have increased their investment in epidemic prevention and implemented a compulsory immunization system, it has promoted the rapid growth of animal vaccine market. At present, many animal vaccine companies still use the original method of preparing broth to prepare vaccine culture medium. The production operation is cumbersome and the stability is not so good. The abuse of antibiotics causes frequent quality problems in fresh animal tissues such as beef, pig liver, and pig stomach, then results in large differences between batches, unstable quality, low number of viable bacteria and high serum consumption. After years of research and development, Angel Microbial Nutrition Research Institute has successfully developed a series of serum-free culture media for vaccine production.

Culture media

Packing size

Application

Fermentation yield

Streptococcus culture medium

30000ml/bag

6bags/carton

Streptococcal vaccines

10 billion cfu/ml

Pasteurella culture medium

30000ml/bag

6bags/carton

Pasteurella vaccines

12 billion cfu/ml

Brucella culture medium

5kg/bag

2bags/carton

Brucella A19, M5 vaccines

60 billion cfu/ml

E. coli culture medium

5kg/bag

2bags/carton

Porcine E. coli and recombinant bacteria vaccine

30 billion cfu/ml

Mycoplasma culture medium

5kg/bag

2bags/carton

Mycoplasma hyopneumoniae and Mycoplasma gallisepticum vaccine

109 ccu/ml

Hemophilus parahaemophilus culture medium

30000ml/bag

6bags/carton

Haemophilus parasuis and Haemophilus paragilloma vaccine

10 billion cfu/ml

Swine erysipelas vaccine culture medium

5kg/bag

2bags/carton

Swine erysipelas vaccine

20 billion cfu/ml

Brain-heart broth

5kg/bag

2bags/carton

For seed culture of microorganisms

Depends

2. Introduction of Angel test medium

Angel test medium is currently mainly used for pipeline microbial detection in large-scale beverage, dairy products, and pharmaceutical companies. It is often used for aseptic verification of PET bottles and aseptic canning production lines. According to Pharmacopoeia and customer’s requirements, and relying on Angel's many years of experience in raw material control of culture media, Angel has developed a variety of culture media for aseptic testing. Angel test medium has upgraded the service of providing customers with a single raw material and give customers better choices.

Culture media

Packing size

Application

Linden Grain Medium (LG)

20kg/bag

The detection of molds, yeasts, heat-resistant molds and the total number of bacteria

Tryptic soy broth (TSB)

5kg/bag

2bags/carton

The enrichment of fastidious bacteria, and aseptic verification of aseptic canning production line

Luria-Bertani broth (LB)

5kg/bag

2bags/carton

The enrichment and fermentation of various E. coli

3. Introduction of Angel culture media for vinegar fermentation

Angel culture media for vinegar fermentation are custom-developed media for the vinegar fermentation industry. They contain salts and growth factors necessary for the growth of acetic acid bacteria. They have the function of strengthening the growth and metabolism of acetic acid bacteria, which can effectively shorten the fermentation period and reduce production cost. This product is food grade with stable quality and strict quality control system

Culture media

Packing size

Application

NS01

7. 5kg/bag

2bags/carton

Fermentation of solid-state brewed vinegar

NS03

7. 5kg/bag

2bags/carton

Fermentation of fruit vinegar, rice vinegar, and spirit vinegar with low acid degree from low alcohol degree

NS04

7. 5kg/bag

2bags/carton

Fermentation of spirit vinegar with high acid degree (above 10 degrees) from high alcohol degree

4. Introduction of Angel culture media for Micro-ecological and Edible Fungus

Angel micro-ecological culture medium is mainly customized for animal probiotics fermentation industry, including lactobacillus medium, bacillus medium, clostridium butyricum medium, photosynthetic bacteria medium, etc.; Angel edible fungus culture medium is mainly customized for seed cultivation of edible fungus and can effectively shorten the fermentation period and reduce production costs.

Culture media

Packing size

Application

Fermentation yield

Lactobacillus medium

20kg/bag

Fermentation of Lactobacillus

Lactobacillus plantarum 10 billion cfu/ml(above), Enterococcus faecium 5 billion cfu/ml(above), Lactobacillus acidophilus 2 billion cfu/ml (above)

Bacillus medium

20kg/bag

Fermentation of Bacillus

Bacillus coagulans 10 billion cfu/ml(above),sporulation rate 90%(above)

Clostridium butyricum medium

5kg/bag

2袋/carton

Fermentation of Clostridiuim butyricum

1 billion cfu/ml(above)

Photosynthetic bacteria medium

5kg/bag

2bags/carton

Fermentation of Photosynthetic bacteria

1 billion cfu/ml(above)

Mushroom medium MP200

5kg/bag

2bags/carton

Fermentation of pleurotus eryngii

High density of mycelial pellets

Mushroom medium MP300

5kg/bag

2bags/carton

Fermentation of various mushroom

High density of mycelial pellets

Mushroom medium(PDA)

5kg/bag

2bags/carton

Seed cultivation and preservation of edible fungi

High density of mycelium


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Published by Chen Lin

R&D expert of Protein Nutrition and Flavoring technology center


About Angel Microbial Nutrition:

Angel YE, rich in protein, amino acids, peptides, nucleotides, B vitamins and trace elements. With the advantages of having no allergic source and being non-transgenic, high efficiency and stable quality, Angel YE are widely applied in the biological laboratories and industrial-scale fermentation, promoting the product safety and technical progress in the global fermentation industry.

About Angel:

Angel Yeast Company is a high-tech listed company specializing in yeast and biotech. Product business covers Yeast and Baking, Yeast Extract-Savoury, Nutrition & Health and Biotechnology fields. It is one of the world's leading companies in the yeast industry. Angel has 12 holding subsidiaries and provides products and services for more than 150 countries and regions.


Press contact:

ANGEL YEAST CO., LTD

Address: 168 Chengdong Avenue, Yichang, Hubei 443003, P. R.China

Tel: +86-717-6369227

Fax: +86-717-6368757

email: aie@angelyeast.com



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